Stereochemistry of polynucleotide formation in enzyme reactions
Summary
Type: HPLC Application #: 107302
Detection: UV, 254nm Conditions: eluent: 0.5M ammonium acetate pH 4.4, for fig. 2 and for elution of the products of the nearest neighbour analysis: 0.25M KCl, 0.05M KH2PO4 pH 4.5 flow rate 1.0ml/min pressure 180 - 220atm Authors: Burgers PMJ Source: Biochemistry 18 (1979) 450 - 454
Details
Legend: Fig. 2. LC analysis of the diastereomers of ADPaS, A (16.5min) Sp, B (19.2min)
Rp diastereomer. Fig. 3. LC analysis of digestion products of the copolymer,
[A] after DEAE Sephadex chromatography (fr